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Journal Articles

I. Spheryx Publications

Tiffany Markus, Juliana Lumer, River Stasavage, David B Ruffner, Laura A Philips, Fook Chiong Cheong, "Monitoring polysorbate 80 degradation in protein solutions using Total Holographic Characterization," I. J. Pharm. 652, 123843 (2024)


Richard E Cavicchi, Laura A Philips, Fook Chiong Cheong, David B Ruffner, Priya Kasimbeg, Wyatt Vreeland, “Distribution of Average Aggregate Density from Stir-Stressed NISTmAb Protein,” J. Pharm. Sci., 111, 1614-1624 (2022).

Rostislav Boltyanskiy, Mary Ann Odete, Fook Chiong Cheong, Laura A Philips, “Label-free viability assay using in-line holographic video microscopy,” Scientific Reports 12, 1-9 (2022)

An interlaboratory comparison on the characterization of a sub-micrometer polydisperse particle dispersion, K. D. Benkstein, G. Balakrishnan, A. Bhirde, P. Chalus, T. K. Das, N. Do, D. L. Duewer, N. Filonsov, F. C. Cheong, P. Garidel, N. S. Gill, A. D. Grabarek, D. G. Grier, J. Hadley, A. D. Hollingsworth, W. W. Howard, M. Jarzebski, W. Jiskoot, S. R. Kar, V. Kestens, H. Khasa, Y. J. Kim, A. Koulov, A. Matter, L. A. Philips, C. Probst, Y. Ramaye, T. W. Randolph, D. C. Ripple, S. Romeijn, M. Saggu, F. Schleinzer, J. R. Snell, J. K. Tatarkiewicz, H. A. Wright and D. Yang, An Interlaboratory Comparison on the Characterization of a Sub-micrometer Polydisperse Particle Dispersion,“ J. Pharm. Sci. 111, 699-709 (2022).

L. E. Altman, R. Quddus, F. C. Cheong and D. G. Grier, “Holographic characterization and tracking of colloidal dimers in the effective-sphere approximation,” Soft Matter 17, 2695-2703 (2021).

Mary Ann Odete, Fook Chiong Cheong, Annemarie Winters, Jesse J. Elliott, Laura A. Philips and David G. Grier, "The role of the medium in the effective-sphere interpretation of holographic particle characterization data," Soft Matter, 16, 891-898 (2020).

Annemarie Winters, Fook Chiong Cheong, Mary Ann Odete, Juliana Lumer, David B. Ruffner, Kimberly I. Mishra, David G. Grier, Laura A. Philips, “Quantitative Differentiation of Protein Aggregates from Other Subvisible Particles in Viscous Mixtures Through Holographic Characterization," J. Pharm. Sci., 109, 2405-2412 (2020).

Priya Kasimbeg, Fook Chiong Cheong, David B. Ruffner, Jaroslaw M. Blusewicz, and Laura A. Philips, “Holographic Characterization of Protein Aggregates in the Presence of Silicone Oil and Surfactants,” J. Pharm. Sci., 108, 155-161 (2019).

David B. Ruffner, Fook Chiong Cheong, Jaroslaw M. Blusewicz, and Laura A. Philips, "Lifting degeneracy in holographic characterization of colloidal particles using multi-color imaging," Opt. Express 26, 13239-13251 (2018).

Fook Chiong Cheong, Priya Kasimbeg, Annemarie Winters, Jaroslaw M. Blusewicz, Ei-Hnin Hlaing, David B. Ruffner, Robin V. Ihnfeldt, David G. Grier, Laura A. Philips, “Holographic Characterization of Agglomerates in CMP Slurries,” ICPT 2018, October 15-17, 2018, Seoul, Republic of Korea.

Fook Chiong Cheong, Priya Kasimbeg, David B. Ruffner, Ei Hnin Hlaing, Jaroslaw M. Blusewicz, Laura A. Philips, and David G. Grier, “Holographic characterization of colloidal particles in turbid media,” Applied Physics Letters 2017, 111:15.

Laura A. Philips, David B. Ruffner, Fook Chiong Cheong, Jaroslaw M. Blusewicz, Priya Kasimbeg, Basma Waisi, Jeffrey R. McCutcheon, David G. Grier, “Holographic characterization of contaminants in water: Differentiation of suspended particles in heterogeneous dispersions,” Water Research, Volume 122, 2017, Pages 431-439.

Chen Wang, Xiao Zhong, David B. Ruffner, Alexandra Stutt, Laura A. Philips, Michael D. Ward, David G. Grier, “Holographic Characterization of Protein Aggregates”, J. Pharm. Sci., 105, 1074-1085, (2016).

C. Wang, F.C. Cheong, D.B. Ruffner, X. Zhong, M.D. Ward, D.G. Grier, “Holographic Characterization of Colloidal Fractal Aggregates”, Soft Matter, 12, 8774-8780, (2016).

II. Spheryx Customer Publications

Wenjun Chen, Lucas Sixdenier, Angus McMullen, David G. Grier and Jasna Brujic of New York University, "Refractive-index and density-matched emulsions with programmable DNA interactions," Soft Matter 2024 Advance Article (2024)


Wei Chen, Denis Klemm, Kathrin Gregoritza, Ravuri Satya Krishna Kishore, Jan Olaf Stracke, Christine Worth, Cosimo Pinto and Nuria Sancho Oltra of F. Hoffmann-La Roche AG, Basel, Switzerland, Screening Techniques for Monitoring the Sub-visible Particle Formation of Free Fatty Acids in Biopharmaceuticals," European Journal of Pharmaceutics and Biopharmaceutics 190, 242-247 (2023)

Harri Rahn, Merve Oeztuerk, Nikolai Hentz, Friedrike Junge and Markus Hollmann of AbbVie Deutschland GmbH & Co, "The Strengths of Total Holographic Video Microscopy in detecting sub-visible protein particles in Biopharmaceuticals: A comparison to Flow Imaging and Resonant Measurement," Journal of Pharmaceutical Sciences 112, 985-990 (2023)

Takano Shin, Ono Ryohei ,Sakurai Kazuo, "A surprisingly narrow particle size distribution for polyacrylic acid nanospheres produced by precipitation polymerization and revealed by small-angle X-ray scattering," Polymer Journal,Volume 55, Issue 12, 1387-1391 (2023)

Nishida C, Tomonaga T, Izumi H, Wang KY, Higashi H, Ishidao T, Takeshita JI, Ono R, Sumiya K, Fujii S, Mochizuki S, Sakurai K, Yamasaki K, Yatera K, Morimoto Y., "Inflammogenic effect of polyacrylic acid in rat lung following intratracheal instillation," Particle and Fibre Toxicology 19, 8 (2022)

Jae Seon Baik, Sanga Kim, Dae-Woong Jung, Weon-Sik Chae, Changhyun Pang, Suk Ho Bhang, Laurent Corte, Gi-Ra Yi*, "Colloidal Supraballs of Mesoporous Silica Nanoparticles as Bioresorbable Adhesives for Hydrogels," Chemistry of Materials 34, 548-593 (2022)

Jeong-Bin Moon, Seung-Jea Lee, Seung-Hyun Kim, Yechan Chang, Hyerim Hwang, Gi-Ra Yi*, "Mie-Resonant Rutile TiO2 Nanospheres for Unclonable Structural Colors," Chemistry of Materials 34, 7897- 7905 (2022).

Altman LE, Quddus R, Cheong FC, Grier DG, "Holographic characterization and tracking of colloidal dimers in the effective-sphere approximation," Soft Matter 17, 2695-2703 (2021).

You-Jin Kim, Jae-Hyun Kim, In-Seong Jo, David J. Pine, Stefano Sacanna, Gi-Ra Yi*,
"Patchy colloidal clusters with broken symmetry," Journal of the American Chemical Society 143, 33, 13175–13183 (2021).

Snyder K, Quddus R, Hollingsworth AD, Kirshenbaum K, Grier DG, "Holographic immunoassays: direct detection of antibodies binding to colloidal spheres," Soft Matter 16, 10180-10186 (2020).

Jo IS, Oh JS, Kim SH, Pine DJ, Yi GR, "Compressible colloidal clusters from Pickering emulsions and their DNA functionalization," Chem Commun 54, 8328-8331 (2018).

II. Real World Applications

Kaitlynn Snyder, Rushna Quddus, Andrew D. Hollingsworth, Kent Kirshenbaum and David G. Grier, “Holographic immunoassays: direct detection of antibodies binding to colloidal spheres,” Soft Matter,2020, 16, 10180-10186 (2020).


Mary Ann Odete, Fook Chiong Cheong, Annemarie Winters, Jesse J. Elliott, Laura A. Philips and David G. Grier, "The role of the medium in the effective-sphere interpretation of holographic particle characterization data," Soft Matter, 16, 891-898 (2020).

Annemarie Winters, Fook Chiong Cheong, Mary Ann Odete, Juliana Lumer, David B. Ruffner, Kimberly I. Mishra, David G. Grier, Laura A. Philips, “Quantitative Differentiation of Protein Aggregates from Other Subvisible Particles in Viscous Mixtures Through Holographic Characterization," J. Pharm. Sci., 109, 2405-2412 (2020).

Priya Kasimbeg, Fook Chiong Cheong, David B. Ruffner, Jaroslaw M. Blusewicz, and Laura A. Philips, “Holographic Characterization of Protein Aggregates in the Presence of Silicone Oil and Surfactants,” J. Pharm. Sci., 108, 155-161 (2019).

Middleton C, Hannel MD, Hollingsworth AD, Pine DJ, Grier DG, "Optimizing the synthesis of monodisperse colloidal spheres using holographic particle characterization," Langmuir 35, 6602-6609 (2019)

Fook Chiong Cheong, Priya Kasimbeg, Annemarie Winters, Jaroslaw M. Blusewicz, Ei-Hnin Hlaing, David B. Ruffner, Robin V. Ihnfeldt, David G. Grier, Laura A. Philips, “Holographic Characterization of Agglomerates in CMP Slurries,” ICPT 2018, October 15-17, 2018, Seoul, Republic of Korea.

Fook Chiong Cheong, Priya Kasimbeg, David B. Ruffner, Ei Hnin Hlaing, Jaroslaw M. Blusewicz, Laura A. Philips, and David G. Grier, “Holographic characterization of colloidal particles in turbid media,” Applied Physics Letters 2017, 111:15

Laura A. Philips, David B. Ruffner, Fook Chiong Cheong, Jaroslaw M. Blusewicz, Priya Kasimbeg, Basma Waisi, Jeffrey R. McCutcheon, David G. Grier, “Holographic characterization of contaminants in water: Differentiation of suspended particles in heterogeneous dispersions,” Water Research, Volume 122, 2017, Pages 431-439

Chen Wang, Xiao Zhong, David B. Ruffner, Alexandra Stutt, Laura A. Philips, Michael D. Ward, David G. Grier, “Holographic Characterization of Protein Aggregates”, J. Pharm. Sci., 105, 1074-1085, (2016).

C. Wang, F.C. Cheong, D.B. Ruffner, X. Zhong, M.D. Ward, D.G. Grier, “Holographic Characterization of Colloidal Fractal Aggregates”, Soft Matter, 12, 8774-8780, (2016).

H. Shpaisman, B. J. Krishnatreya, D. G. Grier, “Holographic microrefractometer,” Appl. Phys. Lett. 101, 091102 (2012).

F. C. Cheong, K. Xiao, D. J. Pine, D. G. Grier, “Holographic characterization of individual colloidal spheres’ porosities,” Soft Matter 7, 6816–6819 (2011).

K. Xiao, D. G. Grier, “Multidimensional optical fractionation with holographic verification,” Phys. Rev. Lett. 104, 028302 (2010).

F. C. Cheong, S. Duarte, S.-H. Lee, D. G. Grier, “Holographic microrheology of polysaccharides from Streptococcus mutans biofilms,” Rheol. Acta 48, 109–115 (2009)

F. C. Cheong, K. Xiao, D. G. Grier, “Characterization of individual milk fat globules with holographic video microscopy,” J. Dairy Sci. 92, 95–99 (2009).

IV. Holographic Video Microscopy

David B. Ruffner, Fook Chiong Cheong, Jaroslaw M. Blusewicz, and Laura A. Philips, "Lifting degeneracy in holographic characterization of colloidal particles using multi-color imaging," Opt. Express 26, 13239-13251 (2018)


C. Wang, H. Shpaisman, A. D. Hollingsworth, D. G. Grier, “Celebrating Soft Matter ’s 10th Anniversary: Monitoring colloidal growth with holographic microscopy,” Soft Matter 11, 1062–1066 (2015).

C. Wang, H. W. Moyses and D. G. Grier, "Stimulus-responsive colloidal sensors with fast holographic readout", Appl. Phys. Lett. 107, 051903 (2015).

M. Hannel, C. Middleton and D. G. Grier, "Holographic characterization of imperfect colloidal spheres", Appl. Phys. Lett. 107, 141905 (2015).

B. J. Krishnatreya, A. Colen-Landy, P. Hasebe, B. A. Bell, J. R. Jones, A. Sunda-Meya, D. G. Grier, “Measuring Boltzmann’s constant through holographic video microscopy of a single sphere,” Am. J. Phys. 82, 23–31 (2014).

H. Moyses, B. J. Krishnatreya, D. G. Grier, “Robustness of holographic video microscopy against defects in illumination,” Opt. Express 21, 5968–5973 (2013).

F. C. Cheong, B. J. Krishnatreya, D. G. Grier, “Strategies for three-dimensional particle tracking with holographic video microscopy,” Opt. Express 18, 13563–13573 (2010).

F. C. Cheong, B. Sun, R. Dreyfus, J. Amato-Grill, K. Xiao, L. Dixon, D. G. Grier, “Flow visualization and flow cytometry with holographic video microscopy,” Opt. Express 17, 13071– 13079 (2009).

S.-H. Lee, Y. Roichman, G.-R. Yi, S.-H. Kim, S.-M. Yang, A. van Blaaderen, P. van Oostrum, D. G. Grier, “Characterizing and tracking single colloidal particles with video holographic microscopy,” Opt. Express 15, 18275–18282 (2007).

V. Other Relevant Publications

L. Dixon, F. C. Cheong, D. G. Grier, “Holographic particle-streak velocimetry,” Opt. Express 19, 4393–4398 (2011).


L. Dixon, F. C. Cheong, D. G. Grier, “Holographic deconvolution microscopy for high- resolution particle tracking,” Opt. Express 19, 16410–16417 (2011).

F. C. Cheong, D. G. Grier, “Rotational and translational diffusion of copper oxide nanorods measured with holographic video microscopy,” Opt. Express 18, 6555–6562 (2010).

S.-H. Lee, D. G. Grier, “Holographic microscopy of holographically trapped three-dimensional structures,” Opt. Express 15, 1505–1512 (2007).

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