Cell viability is a key parameter of interest in many applications in both industry and academia. Yeast cells, for example, are utilized in applications that span protein manufacturing, medical research in genetics, and of course, consumer products. While numerous methods of assessing cell viability exist, most require a staining protocol, which is often time-consuming and unreliable.
Our novel approach to measuring cell viability with Total Holographic Characterization (THC) on xSight offers a fast, reliable, and label-free alternative to existing dye exclusion assays.
Changes in refractive index upon cell death allow xSight to easily distinguish live and dead yeast cells without staining and manual counting.
xSight measurements are consistent with the trypan blue staining exclusion assay:
Fresh Branching budding yeast
Contact us for more information on how Spheryx can help with your cell viability measurements.